Rapid-cycle PCR detection of Pyrenophora graminea from barley seed
نویسندگان
چکیده
منابع مشابه
Rapid nested PCR-based detection of Ramularia collo-cygni direct from barley.
Ramularia collo-cygni is a barley pathogen of increasing importance in Northern and Central Europe, New Zealand and South America. Accurate visual and microscopic identification of the pathogen from diseased tissue is difficult. A nested PCR-based diagnostic test has been developed as part of an initiative to map the distribution of the pathogen in Scotland. The entire nuclear ribosomal interna...
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The usefulness of IRAP (inter-retrotransposon amplified polymorphism) and ITS-RFLP (restriction of PCR-amplified internal transcribed spacers of the rDNA) markers in the analysis of 39 Pyrenophora graminea isolates was determined. Each marker system could discriminate between all of the isolates in detecting polymorphism, albeit with variable efficiency. IRAP and ITS-RFLP produced 85% and 77% p...
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Extended Abstract Introduction and Objective: Leaf stripe disease of barley is caused by the seed transmitted hemi-biotrophic fungus Pyneophera graminea which has spread in to important areas of barley production in Iran especially in Khorasan Razavi province. The fungus survives as a mycelium in the pericarp, the hull and the seed coat, but not in the embryo. During the seed generation, its g...
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ژورنال
عنوان ژورنال: Plant Pathology
سال: 2001
ISSN: 0032-0862
DOI: 10.1046/j.1365-3059.2001.00563.x